SRAMM: Short Read Alignment Mapping Metrics

نویسندگان

چکیده

Short Read Alignment Mapping Metrics (SRAMM): is an efficient and versatile command line tool providing additional short read mapping metrics, filtering, graphs. aligners report MAPing Quality (MAPQ), but these methods generally are neither standardized nor well described in literature or software manuals. Additionally, third party quality programs typically computationally intensive designed for specific applications. SRAMM efficiently generates multiple different concept-based scores to provide informative post alignment examination filtering process of aligned reads various downstream compatible with Python 2.6+ 3.6+ on all operating systems. It works any aligner that SAM/BAM/CRAM file outputs reports 'AS' tags. freely available under the MIT license at http://github.com/achon/sramm.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Improving PacBio Long Read Accuracy by Short Read Alignment

The recent development of third generation sequencing (TGS) generates much longer reads than second generation sequencing (SGS) and thus provides a chance to solve problems that are difficult to study through SGS alone. However, higher raw read error rates are an intrinsic drawback in most TGS technologies. Here we present a computational method, LSC, to perform error correction of TGS long rea...

متن کامل

Short read alignment with populations of genomes

SUMMARY The increasing availability of high-throughput sequencing technologies has led to thousands of human genomes having been sequenced in the past years. Efforts such as the 1000 Genomes Project further add to the availability of human genome variation data. However, to date, there is no method that can map reads of a newly sequenced human genome to a large collection of genomes. Instead, m...

متن کامل

FPGA Acceleration of Short Read Alignment

Aligning millions of short DNA or RNA reads, of 75 to 250 base pairs each, to a reference genome is a significant computation problem in bioinformatics. We present a flexible and fast FPGA-based short read alignment tool. Our aligner makes use of the processing power of FPGAs in conjunction with the greater host memory bandwidth and flexibility of software to improve performance and achieve a h...

متن کامل

SHRiMP2: Sensitive yet Practical Short Read Mapping

UNLABELLED We report on a major update (version 2) of the original SHort Read Mapping Program (SHRiMP). SHRiMP2 primarily targets mapping sensitivity, and is able to achieve high accuracy at a very reasonable speed. SHRiMP2 supports both letter space and color space (AB/SOLiD) reads, enables for direct alignment of paired reads and uses parallel computation to fully utilize multi-core architect...

متن کامل

Sequence analysis GateKeeper: a new hardware architecture for accelerating pre-alignment in DNA short read mapping

Motivation: High throughput DNA sequencing (HTS) technologies generate an excessive number of small DNA segments -called short readsthat cause significant computational burden. To analyze the entire genome, each of the billions of short reads must be mapped to a reference genome based on the similarity between a read and ‘candidate’ locations in that reference genome. The similarity measurement...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: International journal on bioinformatics & biosciences

سال: 2021

ISSN: ['1839-9614']

DOI: https://doi.org/10.5121/ijbb.2021.11201